Use of lentiviral vectors in vaccination
- Category: HIV based Lentiviral Vectors
- Created: Monday, 11 October 2021 16:55
- Written by Min-Wen Ku, Pierre Charneau, Laleh Majlessi
Introduction: Lentiviral vectors have emerged as powerful vectors for vaccination, due to their high efficiency to transduce dendritic cells and to induce long-lasting humoral immunity, CD8+ T cells, and effective protection in numerous preclinical animal models of infection and oncology.
Areas covered: Here, we reviewed the literature, highlighting the relevance of lentiviral vectors in vaccinology. We recapitulated both their virological and immunological aspects of lentiviral vectors. We compared lentiviral vectors to the gold standard viral vaccine vectors, i.e. adenoviral vectors, and updated the latest results in lentiviral vector-based vaccination in preclinical models.
Expert opinion: Lentiviral vectors are non-replicative, negligibly inflammatory, and not targets of preexisting immunity in human populations. These are major characteristics to consider in vaccine development. The potential of lentiviral vectors to transduce non-dividing cells, including dendritic cells, is determinant in their strong immunogenicity. Notably, lentiviral vectors can be engineered to target antigen expression to specific host cells. The very weak inflammatory properties of these vectors allow their use in mucosal vaccination, with particular interest in infectious diseases that affect the lungs or brain, including COVID-19. Recent results in various preclinical models have reinforced the interest of these vectors in prophylaxis against infectious diseases and in onco-immunotherapy.
Keywords: Antigen expression in vivo; humoral immunity; lentiviral vectors; long-lasting immune memory; non-integrating lentiviral vectors; t-cell immunity; transduction; vaccination.
A single immunization with a minute dose of a lentiviral vector-based vaccine is highly effective at eliciting protective humoral immunity against West Nile virus.J Gene Med. 2006 Mar;8(3):265-74. doi: 10.1002/jgm.837.PMID: 16308885
Conventional dendritic cells are required for the activation of helper-dependent CD8 T cell responses to a model antigen after cutaneous vaccination with lentiviral vectors.J Immunol. 2011 Apr 15;186(8):4565-72. doi: 10.4049/jimmunol.1002529. Epub 2011 Mar 9.PMID: 21389256 Free PMC article.
Immunization of mice with lentiviral vectors targeted to MHC class II+ cells is due to preferential transduction of dendritic cells in vivo.PLoS One. 2014 Jul 24;9(7):e101644. doi: 10.1371/journal.pone.0101644. eCollection 2014.PMID: 25058148 Free PMC article.
Protective antiviral immunity conferred by a nonintegrative lentiviral vector-based vaccine.PLoS One. 2008;3(12):e3973. doi: 10.1371/journal.pone.0003973. Epub 2008 Dec 19.PMID: 19096527 Free PMC article.
Lentiviral vectors for immunization: an inflammatory field.Expert Rev Vaccines. 2010 Mar;9(3):309-21. doi: 10.1586/erv.10.9.PMID: 20218859 Review.
- PMID: 34620025
- DOI: 10.1080/14760584.2021.1988854