Ivermectin reverses the drug resistance in cancer cells through EGFR/ERK/Akt/NF-κB pathway
- Details
- Category: Ivermectin
- Created: Tuesday, 18 June 2019 18:15
- Written by Lu Jiang, Pan Wang, Ying-Jian Sun & Yi-Jun Wu - Journal of Experimental & Clinical Cancer Research volume 38, Article number: 265 (2019)
Background
Discovery and development of novel drugs that are capable of overcoming drug resistance in tumor cells are urgently needed clinically. In this study, we sought to explore whether ivermectin (IVM), a macrolide antiparasitic agent, could overcome the resistance of cancer cells to the therapeutic drugs.
Methods
We used two solid tumor cell lines (HCT-8 colorectal cancer cells and MCF-7 breast cancer cells) and one hematologic tumor cell line (K562 chronic myeloid leukemia cells), which are resistant to the chemotherapeutic drugs vincristine and adriamycin respectively, and two xenograft mice models, including the solid tumor model in nude mice with the resistant HCT-8 cells and the leukemia model in NOD/SCID mice with the resistant K562 cells to investigate the reversal effect of IVM on the resistance in vitro and in vivo. MTT assay was used to investigate the effect of IVM on cancer cells growth in vitro. Flow cytometry, immunohistochemistry, and immunofluorescence were performed to investigate the reversal effect of IVM in vivo. Western blotting, qPCR, luciferase reporter assay and ChIP assay were used to detect the molecular mechanism of the reversal effect. Octet RED96 system and Co-IP were used to determine the interactions between IVM and EGFR.
Results
Our results indicated that ivermectin at its very low dose, which did not induce obvious cytotoxicity, drastically reversed the resistance of the tumor cells to the chemotherapeutic drugs both in vitro and in vivo. Mechanistically, ivermectin reversed the resistance mainly by reducing the expression of P-glycoprotein (P-gp) via inhibiting the epidermal growth factor receptor (EGFR), not by directly inhibiting P-gp activity. Ivermectin bound with the extracellular domain of EGFR, which inhibited the activation of EGFR and its downstream signaling cascade ERK/Akt/NF-κB. The inhibition of the transcriptional factor NF-κB led to the reduced P-gp transcription.
Newest lawyer discovered trafficking children for financial gain
- Details
- Category: Lawyers and Solicitors
- Created: Tuesday, 16 August 2016 19:54
- Written by Administrator
Hume Legal - Joanna Jarasevic - picked up another easy score, that over a few months makes ten thousand dollars cash, and cashed right in. Unfortunately this lawyer was not working for the department at the time, and had no right to deliberately tank her clients case which ultimately ended up with the kids sent to foster care till they're eighteen. The case was simple, clear cut and the biggest point on her side, was the Emergency Order for removal of the kids was not only unlawful - it was maladministration at best and corruption at worst.
The cow should have had this case dismissed. Instead she did nothing in almost a month. Not one affidavit. All she did was collude with the department, and used her scare tactics saying "there's heaps of evidence". Our auditors concluded the complete opposite, and to tell her clients to "consent to establishment", was giving up the battle before it started. She had a fantastic opportunity to put some overreaching lazy caseworkers in place and on the spot, and to save other families the same fate she sentenced this family to. But that would have meant doing more work, and getting paid less money in the long run. As most legal aid lawyers state "they don't get paid enough money to do affidavits". For christ sake, how can parents defend themselves when their own solicitors roll over because it's more financially viable.
Regarding the allegations - there wasn't a thing that couldn't be explained and most of their [FACS] dribble was lies. So much, it would make your toes curl, believe me. Fucking dirty child trafficking mutts. NB: This mutt was given enough notice that her clients were now informed of what happens when u CONSENT TO ESTABLISHMENT. They told her No. And she lied in court and said they said yes, making docs not have to even prove one of their single reprehensible lies. They make me sick. The whole lot of them.
Criminal Syndicate exposed by Patrick O’Dea and Dr Russell Pridgeon
- Details
- Category: Australian Antipaedophile Party
- Created: Monday, 10 December 2018 22:07
- Written by John Pridgeon
See the letter below written by Dr Russell Pridgeon, which explains in graphic, accurate detail WHY children in Australia remain unprotected against sexual abuse. I will be there are a few things regarding this issue that you did not know. Read and be APPALLED!!!
The Court hearing for Russell and Patrick ODea is tabled for this Friday at the Brisbane magistrates Court.
PLEASE, EVERYONE IN BRISBANE WHO CARES ABOUT PROTECTING CHILDREN, AND WOULD LIKE TO SEE AN END TO THE TERROR VISITED UPON HUNDREDS OF AUSTRALIAN KIDS, BE THERE.
Paedophile's 'snuff' sex plot revealed
- Details
- Category: Paedophiles
- Created: Sunday, 06 March 2011 21:00
- Written by Yoni Bashan - The Sunday Telegraph
A NOTORIOUS paedophile who allegedly planned to set up a "snuff camp" on his release from jail was freed on a legal technicality that left him unsupervised in the community for almost six months.
The notorious sex offender, who cannot be named for legal reasons, was released from jail after his sentence expired in its entirety on April 23 last year.
He only reappeared on the prison radar after being re-arrested in October for a raft of fraud offences in the Wollongong area of NSW.
Six months before his release, prison intelligence uncovered a set of letters being sent by the inmate to another paedophile, a former scoutmaster, located in a separate wing of Goulburn jail.
The pair had planned to set up a "snuff camp" in country NSW upon their release.
The letters outlined the names, ages and addresses of the child victims they intended to abduct - all were living around the Mudgee and Rylestone areas of NSW.
The two men had secured funding to buy a secluded, rural property to film the videos, and vehicles to assist with transport.
They discussed procedures they would undertake if police ever cottoned on to their plans.
Prior to his release, prison authorities flagged the inmate as being a danger to the public, and applied to keep him behind bars under a Continuing Detention Order (CDO).
What country in the world condones rape of a child age two?
- Details
- Category: Australian Antipaedophile Party
- Created: Tuesday, 20 November 2018 10:34
- Written by Dr Russell Pridgeon - Family Court Hero
The answer is Australia.
It is important that Australians understand the de facto reality that has arisen in Australia, without being noticed or commented upon by anybody: incest is no longer considered to be a crime by the Child Protection authorities, the courts, or the parliaments of this country.
It is the nearest thing to an impossibility to have a child’s allegations of sexual abuse by a family member competently investigated: the authorities will make any excuse not to prosecute the offender.
The letter below was sent to a Federal MP who was reported in the media as being deeply opposed to child sexual abuse: there was no effective response: only a form letter from a secretary:
MP Pru Goward and NSW FACS hiding even more deaths of children in care
- Details
- Category: Child abuse industry deaths
- Created: Saturday, 14 April 2018 22:13
- Written by Alecomm2
Latest reports published by the NSW government are deliberately misleading the amount of children who die under their watch. First Pru Goward blended the deaths of children in foster care with those who were “known to the system”. Now any child placed on a Guardianship Order is not even “classified” as being in out of home care, meaning those statistics have been removed/hidden also
It seems our government will stop at nothing to keep those shiny dollars coming in for the golden stakeholders, whom thrive exponentially whilst obliterating families.
Click here to view the states 2016 Child Deaths Annual Report
We really need to get control freaks out of our government.
- Details
- Category: NSW Victims Stories
- Created: Thursday, 10 November 2016 22:52
- Written by Alecomm2
We really need to get control freaks out of our government. They're supposed to be public servants not Lords and masters. As someone who has suffered the irrational and corrupt way that child services deals with innocent parents I can say with confidence that unless your child is being starved, beaten, hog tied and kept in a dungeon they are better off with you than our crooked child agencies.
The Criminal Syndicate of Child Protectors
- Details
- Category: Australian Antipaedophile Party
- Created: Saturday, 06 April 2019 22:48
- Written by Alecomm2
Wow. The media certainly didn't do any research before publishing this article. How disappointing. If you had the cohuna's you'd do some and then retract all the wording such as criminal syndicate - and go after the courts and the paedophiles that are running rampant particularly in Brisbane Magistrates Court building.
What a bunch of lying criminal poofters. The only people trafficking children are the federal courts, specifically Federal Magistrates like Jarrett and Coker, the criminal children’s representatives (traffickers) and the all-paedophile supporting court psychologists.
Is your child in OOHC ten years old? They can legally apply to come home.
- Details
- Category: Legislation and Acts
- Created: Sunday, 23 October 2016 01:27
- Written by Alecomm2
Children and Young Persons (Care and Protection) Act 1998 No 157 - S51 Duty of Secretary to give information to certain persons
(1) If a child or young person is in the care responsibility of the Secretary under this Part or a warrant issued under section 233, the Secretary:(a) must, as soon as practicable, cause notice of the fact that the child or young person is in the care responsibility of the Secretary, and the fact that an application may be made to the Secretary for the discharge of the child or young person from the care responsibility of the Secretary and the procedures for making such an application, to be given to:(i) in the case of a child who is of or above the age of 10 years or a young person—the child or young person, and
Protecting the Predators and Perpetrators
- Details
- Category: Australian child protection authorities
- Created: Monday, 21 November 2016 20:15
- Written by Julia Zanetti
Police, child protection, family court's, criminal courts, the legal system, our government ... They all protect predators and perpetrators. Hundreds of thousands of stories are emerging Australia wide and yet the general public still don't get it!! No one is exempt, unless you have money to buy your way out!!!
Innocent people are played every single day!! False reports are made, evidence hidden, and when evidence is available it is kept under lock and key!!! Innocent people being forced to plead guilty to crimes they never committed, their children taken under false pretense, and abused in a system that proclaims to provide a better life!! A better life for who?? The money making fraudsters who pocket billions of Australian tax dollars per year?? Or our children??
This is currently what our children endure !! Tell me if this is the better life you all envisioned?? Full of beatings, rape, murder ?? Homelessness, hunger, with no job prospects, little education, no love or true support ?? And ten times the chance of dying ...
Judges must be held accountable for failing to acknowledge and apply risk assessment
- Details
- Category: Paedophile Judges
- Created: Sunday, 04 December 2016 11:58
- Written by Scott Nickells - Fighters Against Child Abuse Australia
The “Man” who raped the 5 year old girl in the toilet block at Orange was set free on bail 1 day before. The judge who gave him bail should be charged as an accessory to her rape !
Yes that might seem a bit extreme to some but we at FACAA are that fed up with judges letting these monsters walk our streets !
The judge who let him out on bail 1 day before he raped that innocent little girl, should have to answer for his/her crimes !
This particular scumbag has been CONVICTED OF (the gods only know how many more he has gotten away with) more than 70 crimes AS AN ADULT !
What is the law and penalties for child abduction and kidnapping charges in NSW?
- Details
- Category: Family court ordered child sexual abuse
- Created: Tuesday, 23 October 2018 11:51
- Written by Sahar Adatia and Jimmy Singh - Criminal Defence Lawyers Australia
Last week, the Australian Federal Police cracked an underground parental abduction ring following a two-year investigation into the group that allegedly helped jilted mothers to abduct and hide their own children across Australia. Three men and a woman were arrested and charged for their alleged role in the kidnapping syndicate, which organised and financed the children’s snatching with plans to even use a yacht to smuggle them to New Zealand. The network managed to evade detection for a decade.
Among those charged include William Russell Massingham Pridgeon, a 64-year-old doctor from Grafton in NSW who is reported to have founded the Australian Anti-Paedophile Party. The party ran senate candidates in the 2016 federal election.
Child abducting basket case or protective mother?
- Details
- Category: Australian Antipaedophile Party
- Created: Thursday, 01 November 2018 22:51
- Written by Alecomm2
Contrary to the untrue claims the paedophile-parent made about his former wife (the protective parent), she was an accomplished business-woman, an active community worker and a loving mother. This protective mother :
- received the Prime Minister’s award for service to the community;
- was a professional businesswoman who owned a multi-disciplinary paediatric allied health practice;
- was recognised with a Rotary Club Paul Harris Fellowship; and,
- kept her and her children’s abuse by her husband private.
There’s much more to this case, in fact the persecution of this mother is a huge slap in the face by all those involved in the recent apology to those who were beaten, raped and sodomised whilst under the care of previous governments.
So before you make judgements or are gullible enough to believe the disproven claims that many mothers make false child sexual abuse claims to obtain “an advantage” in family court, think again. There was no criminal syndicate abducting children, the criminal syndicate is the system that send children to live with their identified abuser. That this is a VERY common occurrence and that there is a tonne of case law judgements backing this up.
More Articles ...
- DoCS aren't damned if they do or damned if they don't remove children
- The Children - Part Two
- Australian Federal Police in the business of protecting paedophiles and failure of public servant to administer duties
- The Children - Part Four
- The Children - Part Three
- The Children - Part One
- AFP dead wrong! Statistics prove jilted mothers are not lying and caselaw proves family courts protect paedophiles
- Children removed from families are at highest risk to suicide
- Senator insists royal commission investigate institution of law; Says family court cover up similar to church and other institutions; Legal system infested with paedophiles and paedophile enablers-2
